VZV IgM ELISA 试剂盒
This 人 VZV IgM ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 VZV IgM.
Quick Overview for VZV IgM ELISA 试剂盒 (ABIN7666553)
抗原
See all VZV IgM products
VZV IgM
(Anti-Varicella Zoster Virus IgM (VZV IgM))
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Plasma, Serum
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原理
- anti-VZV IgM - ELISA kit for detection IgM antibodies to varicella zoster virus (VZV) in human serum (plasma).
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品牌
- ELISA-VIDITEST
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Analytical Method
- Semi-Quantitative
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组件
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- ELISA break-away strips coated with specific antigen STRIPS Ag 1 microplate
- 1.3 mL Calibrator, r.t.u. 1) CAL 1 vial
- 1.3 mL Negative control serum, r.t.u. NC 1 vial
- 1.3 mL Positive control serum, r.t.u. PC 1 vial
- 2 mL RF sorbent2), 25x concentrated RF SORB 25x 1 vial
- 13 mL Anti-human IgM antibodies labelled with horseradish peroxidase r.t.u. (Px-conjugate) CONJ 1 vial
- 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate (TMB substrate), r.t.u. TMB 1 vial
- 13 mL Stop solution, r.t.u. STOP 1 vial
- Instruction manual
- Quality control certificate 1) ready to use 2) Goat anti-human IgG globulin
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试剂未包括
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- anti-VZV IgM assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a native antigen. Anti-VZV antibodies from serum samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away, those that formed complexes with the antigens are later on recognised by animal anti-human IgM antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- VZV IgM (Anti-Varicella Zoster Virus IgM (VZV IgM))
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别名
- VZV IgM
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物质类
- Antibody
抗原 See all VZV IgM products
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