HCP ELISA 试剂盒
This Pichia pastoris HCP ELISA Kit is a Colorimetric ELISA Kit designed to quantify Pichia pastoris HCP.
Quick Overview for HCP ELISA 试剂盒 (ABIN7538041)
抗原
See all HCP products
HCP
(Host Cell Proteins (HCP))
适用
Pichia pastoris
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
6.25 ng/mL - 800 ng/mL
应用范围
ELISA
样品类型
Biological Therapeutics, Cell Culture Supernatant, Purified Protein
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最低检测浓度
- 6.25 ng/mL
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原理
- ELISA Kit for the quantitative Measurement of host cell protein (HCP) residues in biopharmaceuticals by Pichia Pastoris (Strain X-33 and Derivatives) expression systems
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Analytical Method
- Quantitative
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交叉反应 （详细）
- No cross-reaction exists between the homologous antigens.
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灵敏度
- 1.56 ng/mL
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产品特性
- This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.
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组件
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- 1. Pre-Coated Microplate (Detachable)
- 2. Standard (Stock Solution) -500μg/mL
- 3. Detection antibody (100x)
- 4. TMB Substrates
- 5. Stop Solution
- 6. Wash Solution (100x)
- 7. Diluent Buffer (10x)
- 8. Plate Sealer
- 9. Instruction Manual
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试剂未包括
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- 10-1000μL pipettor
- Multichannel pipettor
- 1L sterilized deionized water or ultrarpure water
- Sterilized EP tubes
- Absorbent Paper
- Microplate reader
- High-speed centrifuge
- Mini Centrifuge
- Microplate washer or washing bottles
- Data analysis and graphing software
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Host Cell Proteins (HCP) ELISA Kit
HCP 适用: 大肠杆菌（E. Coli） Colorimetric Sandwich ELISA 2.6 ng/mL – 250.0 ng/mL
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 µL
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实验时间
- 3.5 h
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板类型
- Pre-coated
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实验流程
- This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by in putting the ODvalues of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.
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实验精密度
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Intra-assay precision: 7-11 %
Inter-assay precision: 1.1-5.8 % -
限制
- 仅限研究用
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储存条件
- 4 °C
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- HCP (Host Cell Proteins (HCP))
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别名
- Host Cell Proteins
抗原 See all HCP products
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