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ESRRA ELISA 试剂盒

ESRRA 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN6963248
发货至: 中国
  • 抗原 See all ESRRA ELISA试剂盒
    ESRRA (Estrogen-Related Receptor alpha (ESRRA))
    适用
    • 1
    • 1
    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.16 ng/mL - 10 ng/mL
    最低检测浓度
    0.16 ng/mL
    应用范围
    ELISA
    原理
    The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This kit recognizes Rat ERRα in samples. No Significant cross-reactivity or interference between Rat ERRα and analogues was observed.
    灵敏度
    0.1 ng/mL
    组件
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Reference Standard & Sample Diluent
    • Biotinylated Detection Antibody (100 x concentrate)
    • HRP Conjugate (100 x concentrate)
    • Biotinylated Detection Antibody Diluent
    • HRP Conjugate Diluent
    • Substrate Reagent
    • Stop Solution
    • Wash Buffer (25 x concentrate)
    • Instruction manual
    Featured
    Discover our best selling ESRRA ELISA Kit
  • 样本量
    100 μL
    实验时间
    3.5 h
    板类型
    Pre-coated
    实验流程
    1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
    2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
    3. Aspirate and wash 3 times.
    4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
    5. Aspirate and wash 5 times.
    6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
    7. Add 50 µL Stop Solution. Read at 450 nm immediately.
    8. Calculation of results.
    试剂准备
    1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
    2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
    3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 10 ng/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 10, 5, 2.5, 1.25, 0.63, 0.32, 0.16, 0 ng/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 10 ng/mL working solution to the first tube and mix up to produce a 5 ng/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
    4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
    5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
    样品制备
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ERRα were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ERRα were tested on 3 different plates, 20 replicates in each plate.
    Both intra-CV and inter-CV are < 10 %.
    限制
    仅限研究用
  • 储存条件
    4 °C,-20 °C
    储存方法
    1. For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 6 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
    2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
    有效期
    6 months
  • Yang, Ding, Zhang, Liu, Dong, Tang, Yang, Ma: "Beneficial Effects of Total Phenylethanoid Glycoside Fraction Isolated from Cistanche deserticola on Bone Microstructure in Ovariectomized Rats." in: Oxidative medicine and cellular longevity, Vol. 2019, pp. 2370862, (2020) (PubMed).

  • 抗原 See all ESRRA ELISA试剂盒
    ESRRA (Estrogen-Related Receptor alpha (ESRRA))
    别名
    Estrogen Related Receptor Alpha (ESRRA 产品)
    别名
    err1 ELISA Kit, erra ELISA Kit, esrral ELISA Kit, erralpha ELISA Kit, nr3b1 ELISA Kit, MGC80854 ELISA Kit, ESRRA ELISA Kit, esrl1 ELISA Kit, LOC100125507 ELISA Kit, ERRalpha ELISA Kit, Err1 ELISA Kit, Estrra ELISA Kit, Nr3b1 ELISA Kit, Errra ELISA Kit, ERR1 ELISA Kit, ERRa ELISA Kit, ESRL1 ELISA Kit, NR3B1 ELISA Kit, ESTRRA ELISA Kit, estrogen-related receptor alpha ELISA Kit, estrogen related receptor alpha L homeolog ELISA Kit, estrogen related receptor alpha ELISA Kit, catsper channel auxiliary subunit zeta ELISA Kit, estrogen related receptor, alpha ELISA Kit, esrra ELISA Kit, esrra.L ELISA Kit, ESRRA ELISA Kit, LOC100125507 ELISA Kit, CATSPERZ ELISA Kit, Esrra ELISA Kit
    背景
    ERR1, ERRa, ERRalpha, ESRL1, NR3B1, ESRRA, Nuclear Receptor Subfamily 3, Group B, Member 1, Steroid hormone receptor
    途径
    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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