SARS-CoV-2 N-Protein IgA Antibody ELISA 试剂盒
This 人 和 SARS Coronavirus-2 (SARS-CoV-2) ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 和 SARS Coronavirus-2 (SARS-CoV-2) .
Quick Overview for SARS-CoV-2 N-Protein IgA Antibody ELISA 试剂盒 (ABIN6952758)
抗原
SARS-CoV-2 N-Protein IgA Antibody
适用
人, SARS Coronavirus-2 (SARS-CoV-2)
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Plasma, Serum
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原理
- COVID-19 (SARS-CoV-2) N-Protein Human Antibody Detection Kit (Indirect ELISA method) for the semi-quantitative measurement of human IgA antibody against SARS-CoV-2 in serum or plasma.
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Analytical Method
- Semi-Quantitative
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产品特性
- COVID-19 N-Protein Human IgA ELISA Kit
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组件
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- SARS-CoV-2 N-protein coated 96-well microplate
- Wash Buffer
- Stop Solution
- Assay Diluent
- Positive Control
- HRP-anti positive control IgG Antibody
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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板类型
- Pre-coated
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实验流程
- This COVID19 human IgA antibody ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARSCoV-2 N protein, which combines with the corresponding antibody present in a sample. When a secondary anti-human Antibody-HRP is added, a complex of Antibody-HRPhuman IgA antibody-virus N antigen forms on the microplate. A TMB substrate is added and a blue color is generated. The depth of color is relative to the amount of the anti-SARS-CoV-2 IgA antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL positive control or sample to each well. Incubate 1.5 hours at room temperature.
- Add 100 μL prepared HRP- anti positive control IgG Antibody and/or 100 μL of prepared HRP-anti Human IgG Antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 12 months
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- SARS-CoV-2 N-Protein IgA Antibody
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物质类
- Antibody
抗原
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