SARS-CoV-2 Nucleocapsid ELISA 试剂盒
SARS-CoV-2 N
适用: 人, SARS Coronavirus-2 (SARS-CoV-2)
Colorimetric
Sandwich ELISA
0.07 ng/mL - 50 ng/mL
Cell Culture Supernatant, Serum
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- 抗原 See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) products
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- 适用
- 人, SARS Coronavirus-2 (SARS-CoV-2)
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.07 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.07 ng/mL
- 应用范围
- ELISA
- 原理
- The COVID-19 / SARS-COV-2 Nucleocapsid Protein ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of COVID-19 N Protein in serum (plasma is not recommended in this assay) and cell culture supernatants.
- 样品类型
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects virus COVID-19 N Protein
- 产品特性
- COVID-19 N-Protein ELISA
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
- This assay employs an antibody specific for COVID-19 N Protein coate on a 96-well plate. Standards and samples are pipetted into the wells and COVID-19 N Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-COVID-19 N Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID-19 N Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- 实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Streptavidin solution. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
- 结果分析
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Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 12 months
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- 抗原 See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) products
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- 别名
- SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N 产品)
- 别名
- nucleocapsid phosphoprotein ELISA Kit, N ELISA Kit
- 物质类
- Viral Protein
- 背景
- The spike protein (S-protein) and nucleocapsid protein (N-protein) are encoded by all coronaviruses, including the coronavirus (COVID-19). Nucleocapsid protein is a most abundant protein of coronavirus. The nucleocapsid protein is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. The N protein of coronavirus is chosen as a diagnostic tool since its strong immunogenicity.
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