PSG1 ELISA 试剂盒
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Quick Overview for PSG1 ELISA 试剂盒 (ABIN6951686)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 28.2 pg/mL
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原理
- Human PSG1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects human PSG1. Other species not determined.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Pregnancy Specific beta-1-Glycoprotein 1 (PSG1) ELISA Kit
PSG1 适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Pregnancy Specific beta-1-Glycoprotein 1 (PSG1) ELISA KitPSG1 适用: 人 Colorimetric Competition ELISA 1.0-400 μg/mL Plasma, Serum, Tissue Homogenate
Pregnancy Specific beta-1-Glycoprotein 1 (PSG1) ELISA KitPSG1 适用: 人 AA 35-419 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Pregnancy Specific beta-1-Glycoprotein 1 (PSG1) ELISA KitPSG1 适用: 人 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- PSG1 (Pregnancy Specific beta-1-Glycoprotein 1 (PSG1))
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别名
- PSG1
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背景
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Gene Symbols: B1G1, PSBG1, PSG1, PSGGA
Protein Name (Synonyms): Pregnancy-specific beta-1-glycoprotein 1 (PS-beta-G-1) (PSBG-1) (Pregnancy-specific glycoprotein 1) (CD66 antigen-like family member F) (Fetal liver non-specific cross-reactive antigen 1/2) (FL-NCA-1/2) (PSG95) (Pregnancy-specific beta-1 glycoprotein C/D) (PS-beta-C/D) (CD antigen CD66f)
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基因ID
- 5669
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UniProt
- P11464
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