C1orf33 ELISA 试剂盒
MRTO4
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
- 抗原 See all C1orf33 (MRTO4) products
- C1orf33 (MRTO4) (mRNA Turnover 4 Homolog (MRTO4))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human MRTO4 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 有效期
- 6 months
-
- 抗原 See all C1orf33 (MRTO4) products
- C1orf33 (MRTO4) (mRNA Turnover 4 Homolog (MRTO4))
- 别名
- MRTO4 (MRTO4 产品)
- 别名
- mrt4 ELISA Kit, wu:fb71a02 ELISA Kit, zgc:110388 ELISA Kit, C1orf33 ELISA Kit, MRT4 ELISA Kit, dJ657E11.4 ELISA Kit, RGD1311709 ELISA Kit, 2610012O22Rik ELISA Kit, Mg684 ELISA Kit, Mrt4 ELISA Kit, MRT4 homolog, ribosome maturation factor L homeolog ELISA Kit, MRT4 homolog, ribosome maturation factor ELISA Kit, mRNA turnover 4, ribosome maturation factor ELISA Kit, mrto4.L ELISA Kit, mrto4 ELISA Kit, MRTO4 ELISA Kit, Mrto4 ELISA Kit
- 基因ID
- 51154
- UniProt
- Q9UKD2
-
You are here:
