LIFR ELISA 试剂盒
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- 抗原 See all LIFR ELISA试剂盒
- LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Rat LIFR ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling LIFR ELISA Kit
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- Discover our top product LIFR ELISA Kit
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Leukemia Inhibitory Factor Receptor alpha (LIFR) ELISA Kit
LIFR 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor Receptor alpha (LIFR) ELISA KitLIFR 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor Receptor alpha (LIFR) ELISA KitLIFR 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Leukemia Inhibitory Factor Receptor alpha (LIFR) ELISA KitLIFR 适用: 人 AA 45-833 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all LIFR ELISA试剂盒
- LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))
- 别名
- LIFR (LIFR 产品)
- 别名
- A230075M04Rik ELISA Kit, AW061234 ELISA Kit, LIF ELISA Kit, CD118 ELISA Kit, LIF-R ELISA Kit, SJS2 ELISA Kit, STWS ELISA Kit, SWS ELISA Kit, leukemia inhibitory factor receptor ELISA Kit, LIF receptor alpha ELISA Kit, leukemia inhibitory factor receptor alpha ELISA Kit, Lifr ELISA Kit, LOC397451 ELISA Kit, LIFR ELISA Kit
- 基因ID
- 81680
- UniProt
- O70535
- 途径
- JAK/STAT Signaling, Growth Factor Binding
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