HSD17B14 IQ-ELISA 试剂盒
This 人 HSD17B14 ELISA Kit is a qPCR ELISA Kit designed to quantify 人 HSD17B14.
Quick Overview for HSD17B14 IQ-ELISA 试剂盒 (ABIN6951568)
抗原
See all HSD17B14 IQ-ELISA Kits
HSD17B14
(Hydroxysteroid (17-Beta) Dehydrogenase 14 (HSD17B14))
适用
人
检测方法
qPCR
实验类型
DNA-Binding ELISA
检测范围
1.22 pg/mL - 5000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma, Serum
-
-
最低检测浓度
- 1.22 pg/mL
-
原理
- Human Immunoquantitative (PCR-Based) HSD17B14 ELISA Kit for cell culture supernatants, plasma, and serum samples.
-
Analytical Method
- Quantitative
-
组件
-
- HSD17B14 Microplate (Item A): 96 well PCR plate coated with anti-Human HSD17B14
- Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
- Standards (Item C): 2 vials of recombinant Human HSD17B14
- Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
- Detection Affinity Reagent for HSD17B14 (Item F): 2 vials of a 4x concentrated solution of anti-Human HSD17B14 affinity reagent
- IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
- Primer Solution (Item I): 1.7 ml vial
- PCR Master Mix (Item J): 1.2 ml vial
- PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
- Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
-
试剂未包括
-
- Real-time PCR instrument, Bio-Rad recommended
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for data analysis
- Tubes to prepare standard or sample dilutions
- Heating block or water bath capable of 80°C
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed
- Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
- Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
- Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
- Add 15μL Primer solution 10μL of PCR master mix to each well
- Run real-time PCR
-
试剂准备
-
Recommended Dilution for serum and plasma samples2 - 20 fold
-
限制
- 仅限研究用
-
-
-
有效期
- 6 months
-
-
- HSD17B14 (Hydroxysteroid (17-Beta) Dehydrogenase 14 (HSD17B14))
-
别名
- HSD17B14
-
基因ID
- 51171
-
UniProt
- Q9BPX1
抗原 See all HSD17B14 IQ-ELISA Kits
-
You are here:
