HSF1 ELISA 试剂盒
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- 抗原 See all HSF1 ELISA试剂盒
- HSF1 (Heat Shock Factor Protein 1 (HSF1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human HSF1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product HSF1 ELISA Kit
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Heat Shock Factor Protein 1 (HSF1) ELISA Kit
HSF1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Heat Shock Factor Protein 1 (HSF1) ELISA KitHSF1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Heat Shock Factor Protein 1 (HSF1) ELISA KitHSF1 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Heat Shock Factor Protein 1 (HSF1) ELISA KitHSF1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Heat Shock Factor Protein 1 (HSF1) ELISA KitHSF1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Heat Shock Factor Protein 1 (HSF1) ELISA KitHSF1 适用: 人 Colorimetric Sandwich ELISA 0.625-20 ng/mL Cell Culture Cells, Tissue Lysate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all HSF1 ELISA试剂盒
- HSF1 (Heat Shock Factor Protein 1 (HSF1))
- 别名
- HSF1 (HSF1 产品)
- 别名
- hsf1 ELISA Kit, Hsf ELISA Kit, xhsf1 ELISA Kit, AA960185 ELISA Kit, HSTF1 ELISA Kit, HSF1 ELISA Kit, HSF ELISA Kit, HSF1c ELISA Kit, hsf1a ELISA Kit, hsf1b ELISA Kit, zHSF1a ELISA Kit, zHSF1b ELISA Kit, hstf1 ELISA Kit, heat shock factor ELISA Kit, Heat Shock Factor ELISA Kit, heat shock factor 1 ELISA Kit, heat shock transcription factor 1 ELISA Kit, heat shock transcription factor 1 S homeolog ELISA Kit, hsf1 ELISA Kit, hsf-1 ELISA Kit, Hsf1 ELISA Kit, HSF1 ELISA Kit, hsf1.S ELISA Kit
- 基因ID
- 3297
- UniProt
- Q00613
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