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IGF2 ELISA 试剂盒

This Colorimetric ELISA kit is designed for the quantitative measurement of Pig IGF2.

产品编号 ABIN6951458

发货至: 中国

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Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IGF2 ELISA 试剂盒 (ABIN6951458)

抗原

See all IGF2 ELISA试剂盒

IGF2 (Insulin-Like Growth Factor 2 (IGF2))

适用

Pig

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

2.77 ng/mL - 500 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum

Discover our top product IGF2 ELISA Kit

最低检测浓度

2.77 ng/mL

原理

The Porcine IGF-2 ELISA Kit is a pre-coated sandwich-based assay kit designed to quantitatively measure IGF-2 (IGF2) levels in porcine (pig) cell culture supernatants, plasma, and serum.

Analytical Method

Quantitative

特异性

This ELISA antibody pair detects porcine IGF-2. Other species not determined.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
- Adhesive Sealing Film
试剂未包括
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard or sample dilutions
- Plastic wrap
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应用备注

Suggested Plasma Dilution: 2 fold

样本量

100 μL

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
抗原 See all IGF2 ELISA试剂盒

IGF2 (Insulin-Like Growth Factor 2 (IGF2))

别名

IGF-2

背景

Gene Symbols: IGF2
Protein Name (Synonyms): Insulin-like growth factor II (IGF-II) [Cleaved into: Insulin-like growth factor

基因ID

396916

UniProt

P23695

途径

Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation