PPIL2 ELISA 试剂盒
This Human PPIL2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human PPIL2 in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate.
Quick Overview for PPIL2 ELISA 试剂盒 (ABIN6951429)
抗原
See all PPIL2 products
PPIL2
(Peptidylprolyl Isomerase (Cyclophilin)-Like 2 (PPIL2))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
原理
- Human PPIL2 ELISA Kit.
-
Analytical Method
- Quantitative
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
限制
- 仅限研究用
-
-
-
有效期
- 6 months
-
-
- PPIL2 (Peptidylprolyl Isomerase (Cyclophilin)-Like 2 (PPIL2))
-
别名
- PPIL2
-
基因ID
- 23759
-
UniProt
- Q13356
抗原 See all PPIL2 products
-
You are here:
