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Acetylcholinesterase ELISA 试剂盒

AChE 适用: 小鼠 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951374
发货至: 中国
  • 抗原 See all Acetylcholinesterase (AChE) ELISA试剂盒
    Acetylcholinesterase (AChE)
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Mouse ACHE ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product AChE ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all Acetylcholinesterase (AChE) ELISA试剂盒
    Acetylcholinesterase (AChE)
    别名
    ACHE (AChE 产品)
    别名
    ACEE ELISA Kit, ARACHE ELISA Kit, N-ACHE ELISA Kit, YT ELISA Kit, Ache ELISA Kit, GB14873 ELISA Kit, zgc:92550 ELISA Kit, ACE ELISA Kit, Dsim\\GD20515 ELISA Kit, GD20515 ELISA Kit, dsim_GLEANR_4292 ELISA Kit, ache ELISA Kit, mE1a ELISA Kit, mE1b ELISA Kit, mE1c ELISA Kit, mE1c-long ELISA Kit, mE1d ELISA Kit, mE1d' ELISA Kit, mE1e ELISA Kit, arache ELISA Kit, n-ache ELISA Kit, acetylcholinesterase (Cartwright blood group) ELISA Kit, acetylcholinesterase 2 ELISA Kit, acetylcholinesterase ELISA Kit, Acetylcholine esterase ELISA Kit, acetylcholinesterase (Cartwright blood group) L homeolog ELISA Kit, collagen type I alpha 2 chain ELISA Kit, ACHE ELISA Kit, AChE-2 ELISA Kit, Ache ELISA Kit, ache ELISA Kit, Dsim\Ace ELISA Kit, ache.L ELISA Kit, COL1A2 ELISA Kit, ACE-1 ELISA Kit
    基因ID
    11423
    UniProt
    P21836
    途径
    Skeletal Muscle Fiber Development
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