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RBP4 ELISA 试剂盒

RBP4 适用: 大鼠 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951219
发货至: 中国
  • 抗原 See all RBP4 ELISA试剂盒
    RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
    适用
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    大鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Rat RBP4 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product RBP4 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all RBP4 ELISA试剂盒
    RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
    别名
    RBP4 (RBP4 产品)
    别名
    RDCCAS ELISA Kit, RBP ELISA Kit, RNBP ELISA Kit, rbp4 ELISA Kit, MGC54038 ELISA Kit, srbp ELISA Kit, PRBP ELISA Kit, RBPA ELISA Kit, Rbp-4 ELISA Kit, fb23c12 ELISA Kit, rbp ELISA Kit, wu:fb23c12 ELISA Kit, wu:fb58d04 ELISA Kit, wu:fb72b04 ELISA Kit, zgc:86911 ELISA Kit, retinol binding protein 4 ELISA Kit, renin binding protein ELISA Kit, retinol binding protein 4 S homeolog ELISA Kit, retinol binding protein 4 A, plasma ELISA Kit, retinol binding protein 4, plasma ELISA Kit, retinol binding protein 4 L homeolog ELISA Kit, RBP4 ELISA Kit, RENBP ELISA Kit, rbp4.S ELISA Kit, rbp4 ELISA Kit, Rbp4 ELISA Kit, RBP4A ELISA Kit, rbp4.L ELISA Kit
    基因ID
    25703
    UniProt
    P04916
    途径
    Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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