CHD7 ELISA 试剂盒
CHD7
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all CHD7 products
- CHD7 (Chromodomain Helicase DNA Binding Protein 7 (CHD7))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human CHD7 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all CHD7 products
- CHD7 (Chromodomain Helicase DNA Binding Protein 7 (CHD7))
- 别名
- CHD7 (CHD7 产品)
- 别名
- CHD7 ELISA Kit, fd19h06 ELISA Kit, si:ch211-197o6.2 ELISA Kit, wu:cegs2051 ELISA Kit, wu:fb37f10 ELISA Kit, wu:fb39h04 ELISA Kit, wu:fd19h06 ELISA Kit, HH5 ELISA Kit, IS3 ELISA Kit, KAL5 ELISA Kit, A730019I05Rik ELISA Kit, Cycn ELISA Kit, Cyn ELISA Kit, Dz ELISA Kit, Edy ELISA Kit, Flo ELISA Kit, GENA 47 ELISA Kit, GENA 60 ELISA Kit, Gena 52 ELISA Kit, Lda ELISA Kit, Mt ELISA Kit, Obt ELISA Kit, Todo ELISA Kit, WBE1 ELISA Kit, Whi ELISA Kit, metis ELISA Kit, chromodomain helicase DNA binding protein 7 L homeolog ELISA Kit, chromodomain helicase DNA binding protein 7 ELISA Kit, chd7.L ELISA Kit, CHD7 ELISA Kit, chd7 ELISA Kit, Chd7 ELISA Kit
- 基因ID
- 55636
- UniProt
- Q9P2D1
- 途径
- Sensory Perception of Sound, Chromatin Binding
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