SAE1 ELISA 试剂盒
SAE1
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all SAE1 ELISA试剂盒
- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human SAE1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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SUMO1 Activating Enzyme Subunit 1 (SAE1) ELISA Kit
SAE1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
-
- 抗原 See all SAE1 ELISA试剂盒
- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
- 别名
- SAE1 (SAE1 产品)
- 别名
- AOS1 ELISA Kit, HSPC140 ELISA Kit, SUA1 ELISA Kit, UBLE1A ELISA Kit, 2400010M20Rik ELISA Kit, 2610044L12Rik ELISA Kit, AL033372 ELISA Kit, AW743391 ELISA Kit, D7Ertd177e ELISA Kit, Sua1 ELISA Kit, Uble1a ELISA Kit, Aos1p ELISA Kit, Sua1p ELISA Kit, aos ELISA Kit, sae2a ELISA Kit, uble1a ELISA Kit, wu:fa28b04 ELISA Kit, zgc:86633 ELISA Kit, SUMO1 activating enzyme subunit 1 ELISA Kit, SUMO1 activating enzyme subunit 1 L homeolog ELISA Kit, SAE1 ELISA Kit, Sae1 ELISA Kit, sae1.L ELISA Kit, sae1 ELISA Kit
- 基因ID
- 10055
- UniProt
- Q9UBE0
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