alpha Tubulin ELISA 试剂盒
TUBA1
适用: 人, 小鼠, 大鼠
acetylated
Colorimetric
Sandwich ELISA
Cell Lysate
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- 抗原 See all alpha Tubulin (TUBA1) ELISA试剂盒
- alpha Tubulin (TUBA1)
- 抗原表位
- acetylated
- 适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human, Mouse, Rat Acetylated-Alpha Tubulin and Alpha Tubulin ELISA Kit. This ELISA is for measuring Acetylated-Alpha Tubulin in Human, Mouse, Rat cell lysates.
- 样品类型
- Cell Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human///Mouse///Rat Acetylated-Alpha Tubulin.
- 产品特性
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- Simultaneously measure cleaved protein and pan protein in one experiment (for normalization purpose)
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-cleaved Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Top Product
- Discover our top product TUBA1 ELISA Kit
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all alpha Tubulin (TUBA1) ELISA试剂盒
- alpha Tubulin (TUBA1)
- 别名
- Alpha Tubulin (TUBA1 产品)
- 别名
- Tuba1 ELISA Kit, K-ALPHA-1 ELISA Kit, Tuba-1 ELISA Kit, cb944 ELISA Kit, fb22g06 ELISA Kit, tuba1 ELISA Kit, wu:fb22g06 ELISA Kit, Calpha1 ELISA Kit, tubulin, alpha 1A ELISA Kit, tubulin alpha 1b ELISA Kit, tubulin, alpha 1b ELISA Kit, tubulin, alpha 1B ELISA Kit, Tuba1a ELISA Kit, TUBA1B ELISA Kit, tuba1b ELISA Kit, Tuba1b ELISA Kit
- 基因ID
- 7846
- UniProt
- Q71U36
- 途径
- Microtubule Dynamics
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