FRS2 ELISA 试剂盒
-
- 抗原 See all FRS2 ELISA试剂盒
- FRS2 (Fibroblast Growth Factor Receptor Substrate 2 (FRS2))
- 抗原表位
- pTyr
-
适用
- 人, 小鼠, 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human, Mouse and Rat Phosphotyrosine FRS2 ELISA Kit. This assay semi-quantitatively measures tyrosine-phosphorylated FRS2 in cell lysate samples.
- 样品类型
- Cell Samples, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- This ELISA kit recognizes human FRS2 phosphotyrosine.
- 产品特性
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Featured
- Discover our best selling FRS2 ELISA Kit
- Top Product
- Discover our top product FRS2 ELISA Kit
-
Fibroblast Growth Factor Receptor Substrate 2 (FRS2) ELISA Kit
FRS2 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor Receptor Substrate 2 (FRS2) ELISA KitFRS2 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
-
- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
-
- 抗原 See all FRS2 ELISA试剂盒
- FRS2 (Fibroblast Growth Factor Receptor Substrate 2 (FRS2))
- 别名
- FRS2 (FRS2 产品)
- 别名
- frs2 ELISA Kit, wu:fd21h10 ELISA Kit, zgc:91973 ELISA Kit, FRS2A ELISA Kit, FRS2alpha ELISA Kit, SNT ELISA Kit, SNT-1 ELISA Kit, SNT1 ELISA Kit, 4732458E18 ELISA Kit, C330018A15Rik ELISA Kit, Frs2alpha ELISA Kit, fibroblast growth factor receptor substrate 2 ELISA Kit, fibroblast growth factor receptor substrate 2b ELISA Kit, FRS2 ELISA Kit, frs2b ELISA Kit, Frs2 ELISA Kit
- 基因ID
- 10818
- UniProt
- Q8WU20, Q8C180
- 途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Signaling Events mediated by VEGFR1 and VEGFR2
-
