Hemoglobin Subunit beta ELISA 试剂盒
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Quick Overview for Hemoglobin Subunit beta ELISA 试剂盒 (ABIN6810489)
抗原
See all Hemoglobin Subunit beta (HBB) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.85 ng/mL
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原理
- Human HbA1c/HBB ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects human HBA1c. Other species not determined.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Hemoglobin beta (HBB) ELISA Kit
HBB 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Red Blood Cell Lysate
Hemoglobin beta (HBB) ELISA KitHBB 适用: 人 Colorimetric Competition ELISA 6.17 μg/mL - 500 μg/mL Erythrocyte Lysates, Plasma, Serum
Hemoglobin beta (HBB) ELISA KitHBB 适用: 人 Colorimetric Competition ELISA 1.56 μg/mL - 100 μg/mL Cell Lysate, Plasma, Serum
Hemoglobin beta (HBB) ELISA KitHBB 适用: 人 Colorimetric Competition ELISA 0.12-500 ng/mL Plasma, Red Blood Cell Lysate, Serum
Hemoglobin beta (HBB) ELISA KitHBB 适用: 猴 Colorimetric Sandwich ELISA 0.12-500 ng/mL Red Blood Cell Lysate
Hemoglobin beta (HBB) ELISA KitHBB 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Red Blood Cell Lysate
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))
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别名
- HBB
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物质类
- Viral Protein
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背景
- Gene Symbols: Hemoglobin subunit beta (Beta-globin) (Hemoglobin beta chain) [Cleaved into: LVV-hemorphin
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UniProt
- P68871
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