ACTH ELISA 试剂盒
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北京 101111
Quick Overview for ACTH ELISA 试剂盒 (ABIN6730843)
抗原
See all ACTH ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.1 ng/mL
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原理
- Human/Mouse/Rat Adrenocorticotropic Hormone EIA Kit optimized for serum and cell culture medium. Competition-based ELISA
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Analytical Method
- Quantitative
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特异性
- This EIA kit is designed to detect human, mouse, and rat ACTH
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灵敏度
- 0.5 ng/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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Adrenocorticotropic hormone (ACTH) ELISA Kit
ACTH 适用: 小鼠 Colorimetric Sandwich ELISA 18.75 pg/mL - 1200 pg/mL Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 人 Colorimetric Competition ELISA 1.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 小鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 大鼠 Colorimetric Competition ELISA 1.23 pg/mL - 100 pg/mL Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 小鼠 Colorimetric Competition ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 大鼠 Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 小鼠 Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: 人 Colorimetric Competition ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH 适用: Various Species Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: Human: 2X / Mouse: 2X / Rat: 2X
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
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试剂准备
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Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Standard, Biotinylated ACTH peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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有效期
- 6 months
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- ACTH (Adrenocorticotropic hormone (ACTH))
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别名
- Adrenocorticotropic hormone
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物质类
- Hormone
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背景
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Gene Symbols: POMC
Background: Adrenocorticotropic hormone (ACTH, also adrenocorticotropin, corticotropin) is a 39 amino acids polypeptide tropic hormone. ACTH is synthesized from pre-pro-opiomelanocortin (pre-POMC), which is proteolytically cleaved by endopeptidases to yield various polypeptide fragments with varying physiological activity. ACTH is secreted by the anterior pituitary gland. It stimulates secretion of glucocorticoid steroid hormones from adrenal cortex cells, especially in the zona fasciculata of the adrenal glands. It influences steroid hormone secretion by both rapid short-term mechanisms and long-term actions. ACTH is also used as a medication and diagnostic agent.
Protein Name (Synonyms): Pro-opiomelanocortin (POMC) (Corticotropin-lipotropin) [Cleaved into: NPP, Melanotropin gamma (Gamma-MSH), Potential peptide, Corticotropin (Adrenocorticotropic hormone) (ACTH), Melanotropin alpha (Alpha-MSH), Corticotropin-like intermediary peptide (CLIP), Lipotropin beta (Beta-LPH), Lipotropin gamma (Gamma-LPH), Melanotropin beta (Beta-MSH), Beta-endorphin, Met-enkephalin]
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基因ID
- 5443
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UniProt
- P01189
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途径
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
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