CHRNB3 ELISA 试剂盒
CHRNB3
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all CHRNB3 ELISA试剂盒
- CHRNB3 (Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human CHRNB3 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human CHRNB3.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product CHRNB3 ELISA Kit
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Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3) ELISA Kit
CHRNB3 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all CHRNB3 ELISA试剂盒
- CHRNB3 (Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3))
- 别名
- CHRNB3 (CHRNB3 产品)
- 别名
- 5730417K16Rik ELISA Kit, Acrb3 ELISA Kit, NACHR ELISA Kit, CHRNB3 ELISA Kit, chrnb3 ELISA Kit, cholinergic receptor nicotinic beta 3 subunit ELISA Kit, neuronal acetylcholine receptor subunit beta-3 ELISA Kit, cholinergic receptor, nicotinic, beta polypeptide 3 ELISA Kit, cholinergic receptor, nicotinic beta 3 L homeolog ELISA Kit, cholinergic receptor, nicotinic beta 3 ELISA Kit, cholinergic receptor, nicotinic, beta 3b (neuronal) ELISA Kit, cholinergic receptor, nicotinic, beta polypeptide 3a ELISA Kit, CHRNB3 ELISA Kit, CpipJ_CPIJ012638 ELISA Kit, Chrnb3 ELISA Kit, chrnb3.L ELISA Kit, chrnb3 ELISA Kit, chrnb3b ELISA Kit, chrnb3a ELISA Kit
- UniProt
- Q05901
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