UBE2F ELISA 试剂盒
UBE2F
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all UBE2F products
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human UBE2F ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human UBE2F.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all UBE2F products
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- 别名
- UBE2F (UBE2F 产品)
- 别名
- NCE2 ELISA Kit, 2510010F15Rik ELISA Kit, AI851109 ELISA Kit, RGD1307608 ELISA Kit, zgc:77005 ELISA Kit, ubiquitin conjugating enzyme E2 F (putative) ELISA Kit, NEDD8-conjugating enzyme UBE2F ELISA Kit, ubiquitin-conjugating enzyme E2F (putative) ELISA Kit, ubiquitin conjugating enzyme E2F (putative) S homeolog ELISA Kit, UBE2F ELISA Kit, Tsp_01616 ELISA Kit, ube2f ELISA Kit, ube2f.S ELISA Kit, Ube2f ELISA Kit
- UniProt
- Q969M7
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