VSIG2 ELISA 试剂盒
The 人 VSIG2 ELISA Kit (ABIN6730730) is a Colorimetric ELISA Kit designed to quantify 人 VSIG2.
产品编号 ABIN6730730
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for VSIG2 ELISA 试剂盒 (ABIN6730730)
抗原
See all VSIG2 products
VSIG2
(V-Set and Immunoglobulin Domain Containing 2 (VSIG2))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.26 ng/mL - 75 ng/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma, Serum
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最低检测浓度
- 0.26 ng/mL
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原理
- The Human VSIG2 ELISA Kit is a pre-coated sandwich-based assay kit designed to quantitatively measure VSIG2 levels in human cell culture supernatants, plasma, and serum.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects human VSIG2. Other species not determined.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
- Adhesive Sealing Film
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard or sample dilutions
- Plastic wrap
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应用备注
- Suggested Plasma Dilution: 2-10 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- VSIG2 (V-Set and Immunoglobulin Domain Containing 2 (VSIG2))
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别名
- VSIG2
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背景
- Gene Symbols: V-set and immunoglobulin domain-containing protein 2 (Cortical thymocyte-like protein) (CT-like protein)
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UniProt
- Q96IQ7
抗原 See all VSIG2 products
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