EGFL6 ELISA 试剂盒
The 小鼠 EGFL6 ELISA Kit (ABIN6730718) is a Colorimetric ELISA Kit designed to quantify 小鼠 EGFL6.
产品编号 ABIN6730718
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Quick Overview for EGFL6 ELISA 试剂盒 (ABIN6730718)
抗原
See all EGFL6 ELISA试剂盒
EGFL6
(EGF-Like-Domain, Multiple 6 (EGFL6))
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.08 ng/mL - 10 ng/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Plasma, Serum
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最低检测浓度
- 0.08 ng/mL
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原理
- The Mouse EGFL6 ELISA Kit is a pre-coated sandwich-based assay kit designed to quantitatively measure EGFL6 levels in mouse cell culture supernatants, plasma, and serum.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects mouse EGFL6. Other species not determined.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
- Adhesive Sealing Film
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard or sample dilutions
- Plastic wrap
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EGF-Like-Domain, Multiple 6 (EGFL6) ELISA Kit
EGFL6 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
EGF-Like-Domain, Multiple 6 (EGFL6) ELISA KitEGFL6 适用: 小鼠 Colorimetric Sandwich ELISA 93.75-6000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 2 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- EGFL6 (EGF-Like-Domain, Multiple 6 (EGFL6))
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别名
- EGFL6
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背景
- Gene Symbols: Epidermal growth factor-like protein 6 (EGF-like protein 6) (MAM and EGF domains-containing gene protein) (Protein W80)
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UniProt
- Q9JJZ5
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