HSC70 Interacting Protein HIP ELISA 试剂盒
ST13
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all HSC70 Interacting Protein HIP (ST13) products
- HSC70 Interacting Protein HIP (ST13) (Suppression of Tumorigenicity 13 (ST13))
- 适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse Hip ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse Hip.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all HSC70 Interacting Protein HIP (ST13) products
- HSC70 Interacting Protein HIP (ST13) (Suppression of Tumorigenicity 13 (ST13))
- 别名
- Hip (ST13 产品)
- 别名
- CG32789 ELISA Kit, Dmel\\CG32789 ELISA Kit, anon-EST:fe1A9 ELISA Kit, 1110007I03Rik ELISA Kit, 3110002K08Rik ELISA Kit, AW555194 ELISA Kit, HIP ELISA Kit, HOP ELISA Kit, HSPABP ELISA Kit, HSPABP1 ELISA Kit, PRO0786 ELISA Kit, SNC6 ELISA Kit, p48 ELISA Kit, AAG2 ELISA Kit, FAM10A1 ELISA Kit, FAM10A4 ELISA Kit, P48 ELISA Kit, Hsc/Hsp70-interacting protein ELISA Kit, suppression of tumorigenicity 13 ELISA Kit, ST13, Hsp70 interacting protein ELISA Kit, suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein) ELISA Kit, HIP ELISA Kit, St13 ELISA Kit, ST13 ELISA Kit
- UniProt
- Q7TN16
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