GOT1 ELISA 试剂盒
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- 抗原 See all GOT1 ELISA试剂盒
- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human GOT1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human GOT1.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product GOT1 ELISA Kit
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Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA Kit
GOT1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitGOT1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitGOT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitGOT1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitGOT1 适用: 兔 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitGOT1 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitGOT1 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) ELISA KitSmall Sample GOT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all GOT1 ELISA试剂盒
- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
- 别名
- GOT1 (GOT1 产品)
- 别名
- ASTQTL1 ELISA Kit, GIG18 ELISA Kit, cAspAT ELISA Kit, cCAT ELISA Kit, AST ELISA Kit, ISSD ELISA Kit, NSD ELISA Kit, SD ELISA Kit, SIALIN ELISA Kit, SIASD ELISA Kit, SLD ELISA Kit, xr406 ELISA Kit, CASPAT ELISA Kit, ASPARTATE AMINOTRANSFERASE ELISA Kit, ATAAT ELISA Kit, MATERNAL EFFECT EMBRYO ARREST 17 ELISA Kit, MEE17 ELISA Kit, T26C19.9 ELISA Kit, T26C19_9 ELISA Kit, aspartate aminotransferase ELISA Kit, aatA ELISA Kit, 83.t00021 ELISA Kit, AI789014 ELISA Kit, Got-1 ELISA Kit, Aspat ELISA Kit, Gaspat ELISA Kit, AL022787 ELISA Kit, FABP-pm ELISA Kit, Got-2 ELISA Kit, mAspAT ELISA Kit, ASPATA ELISA Kit, glutamic-oxaloacetic transaminase 1 ELISA Kit, solute carrier family 17 member 5 ELISA Kit, glutamic-oxaloacetic transaminase 1 homeolog ELISA Kit, aspartate aminotransferase ELISA Kit, glutamic-oxaloacetic transaminase 1, soluble ELISA Kit, glutamic-oxaloacetic transaminase 2 ELISA Kit, glutamatic-oxaloacetic transaminase 2, mitochondrial ELISA Kit, GOT1 ELISA Kit, SLC17A5 ELISA Kit, got1.S ELISA Kit, AAT ELISA Kit, aspC ELISA Kit, EHI_006080 ELISA Kit, AST2 ELISA Kit, ast2 ELISA Kit, got1 ELISA Kit, Got1 ELISA Kit, GOT2 ELISA Kit, Got2 ELISA Kit
- UniProt
- P17174
- 途径
- Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process
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