TRH ELISA 试剂盒
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Quick Overview for TRH ELISA 试剂盒 (ABIN6730541)
抗原
See all TRH ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.1 ng/mL
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原理
- Human Thyrotropin-releasing hormone EIA Kit optimized for serum and cell culture media. Competition-based ELISA
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Analytical Method
- Quantitative
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特异性
- This EIA kit is designed to detect human Thyrotropin-releasing hormone.
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灵敏度
- 0.6 ng/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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Thyrotropin-Releasing Hormone (TRH) ELISA Kit
TRH 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Thyrotropin-Releasing Hormone (TRH) ELISA KitTRH 适用: 小鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Plasma, Serum
Thyrotropin-Releasing Hormone (TRH) ELISA KitTRH 适用: 小鼠 Colorimetric Sandwich ELISA 2.5 pg/mL - 160 pg/mL Serum
Thyrotropin-Releasing Hormone (TRH) ELISA KitTRH 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 μIU/mL - 10 μIU/mL Plasma, Serum, Tissue Homogenate
Thyrotropin-Releasing Hormone (TRH) ELISA KitTRH 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Lysate, Tissue Homogenate
Thyrotropin-Releasing Hormone (TRH) ELISA KitTRH 适用: 大鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Plasma, Serum
Thyrotropin-Releasing Hormone (TRH) ELISA KitTRH 适用: Pig Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Thyrotropin-Releasing Hormone (TRH) ELISA KitTRH 适用: 大鼠 Colorimetric Sandwich ELISA 0.156-10 μIU/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Thyrotropin-Releasing Hormone (TRH) ELISA KitTRH 适用: 小鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Plasma, Serum
Thyrotropin-Releasing Hormone (TRH) ELISA KitTRH 适用: 人 Colorimetric Sandwich ELISA 2.5-100 pg/mL Plasma, Serum
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应用备注
- Suggested Plasma Dilution: 8X
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
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试剂准备
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Recommended Dilution for serum and plasma samplesHuman: 8x / Mouse: 8x / Rat: 8x
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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有效期
- 6 months
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- TRH (Thyrotropin-Releasing Hormone (TRH))
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别名
- Thyrotropin-releasing hormone
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背景
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Gene Symbols: TRH
Background: Thyrotropin-releasing hormone (TRH), also called thyrotropin-releasing factor (TRF) or thyroliberin, is a releasing hormone, produced by the hypothalamus, that stimulates the release of thyrotropin (thyroid-stimulating hormone or TSH) and prolactin from the anterior pituitary. It is a tripeptidal hormone produced from a precursor protein. TRH has been used clinically for the treatment of spinocerebellar degeneration and disturbance of consciousness in humans. It has been used to test the response of the anterior pituitary gland. TRH has anti-depressant and anti-suicidal properties, and has been shown in mice to be an anti-aging agent.
Protein Name (Synonyms): Pro-thyrotropin-releasing hormone (Pro-TRH) (Prothyroliberin) [Cleaved into: Thyrotropin-releasing hormone (TRH) (Protirelin) (TSH-releasing factor) (Thyroliberin) (Thyrotropin-releasing factor) (TRF)]
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基因ID
- 7200
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UniProt
- P20396
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途径
- Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour
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