ACLY ELISA 试剂盒
-
- 抗原 See all ACLY ELISA试剂盒
- ACLY (ATP Citrate Lyase (ACLY))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human ACLY ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human ACLY.
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling ACLY ELISA Kit
- Top Product
- Discover our top product ACLY ELISA Kit
-
ATP Citrate Lyase (ACLY) ELISA Kit
ACLY 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
ATP Citrate Lyase (ACLY) ELISA KitACLY 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
ATP Citrate Lyase (ACLY) ELISA KitACLY 适用: 人 Colorimetric Sandwich ELISA 1.563 IU/mL - 100 IU/mL Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all ACLY ELISA试剂盒
- ACLY (ATP Citrate Lyase (ACLY))
- 别名
- ACLY (ACLY 产品)
- 别名
- ACLY ELISA Kit, DDBDRAFT_0205389 ELISA Kit, DDBDRAFT_0235360 ELISA Kit, DDB_0205389 ELISA Kit, DDB_0235360 ELISA Kit, ACL ELISA Kit, BcDNA:LD21334 ELISA Kit, CG8322 ELISA Kit, DmATPCL ELISA Kit, Dmel\\CG8322 ELISA Kit, anon-WO0140519.179 ELISA Kit, dATPCL ELISA Kit, l(2)01466 ELISA Kit, l(2)k09217 ELISA Kit, n(2)k09217 ELISA Kit, ATPCL ELISA Kit, CLATP ELISA Kit, A730098H14Rik ELISA Kit, AW538652 ELISA Kit, Clatp ELISA Kit, acly ELISA Kit, cb722 ELISA Kit, zgc:92008 ELISA Kit, ATP citrate lyase ELISA Kit, ATP citrate lyase S homeolog ELISA Kit, ATP-citrate synthase ELISA Kit, citrate synthase, mitochondrial ELISA Kit, atp-citrate synthase ELISA Kit, ATP citrate lyase a ELISA Kit, ACLY ELISA Kit, acly.S ELISA Kit, LOC587157 ELISA Kit, acly ELISA Kit, ATPCL ELISA Kit, LOC5564509 ELISA Kit, CpipJ_CPIJ010291 ELISA Kit, Bm1_26245 ELISA Kit, ACL ELISA Kit, Acly ELISA Kit, aclya ELISA Kit
- UniProt
- P53396
- 途径
- Warburg Effect
-
