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SBDS ELISA 试剂盒

SBDS 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6730287
发货至: 中国
  • 抗原 See all SBDS ELISA试剂盒
    SBDS (Shwachman-Bodian-Diamond Syndrome (SBDS))
    适用
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human SBDS ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human SBDS.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product SBDS ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all SBDS ELISA试剂盒
    SBDS (Shwachman-Bodian-Diamond Syndrome (SBDS))
    别名
    SBDS (SBDS 产品)
    别名
    cgi-97 ELISA Kit, sds ELISA Kit, swds ELISA Kit, zgc:56700 ELISA Kit, SDS ELISA Kit, SWDS ELISA Kit, 4733401P19Rik ELISA Kit, AI836084 ELISA Kit, CGI-97 ELISA Kit, SBDS ribosome assembly guanine nucleotide exchange factor ELISA Kit, hypothetical protein ELISA Kit, SBDS, ribosome maturation factor ELISA Kit, SBDS ribosome assembly guanine nucleotide exchange factor S homeolog ELISA Kit, SBDS ribosome maturation factor ELISA Kit, sbds ELISA Kit, LOAG_04440 ELISA Kit, sbds.S ELISA Kit, SBDS ELISA Kit, Sbds ELISA Kit
    UniProt
    Q9Y3A5
    途径
    Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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