PKLR ELISA 试剂盒
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Quick Overview for PKLR ELISA 试剂盒 (ABIN6730254)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.01 ng/mL
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原理
- The Human PKLR ELISA Kit is a pre-coated sandwich-based assay kit designed to quantitatively measure PKLR levels in human cell culture supernatants, plasma, and serum.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects human PKLR. Other species not determined.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
- Adhesive Sealing Film
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard or sample dilutions
- Plastic wrap
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Pyruvate Kinase, Liver and RBC (PKLR) ELISA Kit
PKLR 适用: 人 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Pyruvate Kinase, Liver and RBC (PKLR) ELISA KitPKLR 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Pyruvate Kinase, Liver and RBC (PKLR) ELISA KitPKLR 适用: 大鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Pyruvate Kinase, Liver and RBC (PKLR) ELISA KitPKLR 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Pyruvate Kinase, Liver and RBC (PKLR) ELISA KitPKLR 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Pyruvate Kinase, Liver and RBC (PKLR) ELISA KitPKLR 适用: 人 AA 47-574 Colorimetric Sandwich ELISA 93.7 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Pyruvate Kinase, Liver and RBC (PKLR) ELISA KitPKLR 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
Pyruvate Kinase, Liver and RBC (PKLR) ELISA KitPKLR 适用: 大鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 2 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
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别名
- PKLR
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背景
- Gene Symbols: Pyruvate kinase PKLR (EC 2.7.1.40) (Pyruvate kinase 1) (Pyruvate kinase isozymes L/R) (R-type/L-type pyruvate kinase) (Red cell/liver pyruvate kinase)
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UniProt
- P30613
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途径
- Ribonucleoside Biosynthetic Process
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