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Integrin beta 3 ELISA 试剂盒

This 人 Integrin beta 3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 Integrin beta 3.

产品编号 ABIN6730101

发货至: 中国

Quick Overview for Integrin beta 3 ELISA 试剂盒 (ABIN6730101)

抗原

See all Integrin beta 3 (ITGB3) ELISA试剂盒

Integrin beta 3 (ITGB3)

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Discover our top product ITGB3 ELISA Kit

原理

Human Integrin beta 3/CD61 ELISA Kit.

Analytical Method

Quantitative

特异性

This ELISA antibody pair recognizes Human Integrin beta 3.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Integrin beta 3 (ITGB3) ELISA Kit
ITGB3 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate

Integrin beta 3 (ITGB3) ELISA Kit
ITGB3 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Integrin beta 3 (ITGB3) ELISA Kit
ITGB3 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate

Integrin beta 3 (ITGB3) ELISA Kit
ITGB3 适用: 人, 小鼠, 大鼠 pTyr785 Colorimetric Cell ELISA Cell Culture Cells

Integrin beta 3 (ITGB3) ELISA Kit
ITGB3 适用: 人, 小鼠, 大鼠 pTyr773 Colorimetric Cell ELISA Cell Culture Cells

应用备注

Optimal working dilution should be determined by the investigator.

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
抗原 See all Integrin beta 3 (ITGB3) ELISA试剂盒

Integrin beta 3 (ITGB3)

别名

Integrin beta 3 (CD61

UniProt

Q9Y6R9

途径

Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex

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