ALPL ELISA 试剂盒
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- 抗原 See all ALPL ELISA试剂盒
- ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse Alkaline Phosphatase/ALPL ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse Alkaline Phosphatase.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product ALPL ELISA Kit
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Alkaline Phosphatase, Liver/bone/kidney (ALPL) ELISA Kit
ALPL 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Alkaline Phosphatase, Liver/bone/kidney (ALPL) ELISA KitALPL 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Alkaline Phosphatase, Liver/bone/kidney (ALPL) ELISA KitALPL 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Alkaline Phosphatase, Liver/bone/kidney (ALPL) ELISA KitALPL 适用: 兔 Colorimetric Sandwich ELISA 0.156 mIU/mL - 10 mIU/mL Plasma, Serum
Alkaline Phosphatase, Liver/bone/kidney (ALPL) ELISA KitALPL 适用: 犬 Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Alkaline Phosphatase, Liver/bone/kidney (ALPL) ELISA KitALPL 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Alkaline Phosphatase, Liver/bone/kidney (ALPL) ELISA KitALPL 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Alkaline Phosphatase, Liver/bone/kidney (ALPL) ELISA KitALPL 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Alkaline Phosphatase, Liver/bone/kidney (ALPL) ELISA KitALPL 适用: 猴 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all ALPL ELISA试剂盒
- ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))
- 别名
- Alkaline Phosphatase (ALPL (ALPL 产品)
- 别名
- ALP ELISA Kit, iap ELISA Kit, alpl ELISA Kit, AP-TNAP ELISA Kit, APTNAP ELISA Kit, HOPS ELISA Kit, TNAP ELISA Kit, TNSALP ELISA Kit, Akp-2 ELISA Kit, Akp2 ELISA Kit, PHOA ELISA Kit, TNS-AP ELISA Kit, alkaline phosphatase, liver/bone/kidney L homeolog ELISA Kit, alkaline phosphatase, liver/bone/kidney ELISA Kit, alkaline phosphatase, intestinal, gene 2 ELISA Kit, alkaline phosphatase, liver/bone/kidney pseudogene ELISA Kit, alpl.L ELISA Kit, ALPL ELISA Kit, alpi.2 ELISA Kit, LOC100031702 ELISA Kit, Alpl ELISA Kit
- UniProt
- P09242
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