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PGD ELISA 试剂盒

PGD 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6730055
发货至: 中国
  • 抗原 See all PGD ELISA试剂盒
    PGD (Phosphogluconate Dehydrogenase (PGD))
    适用
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human PGD ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human PGD.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all PGD ELISA试剂盒
    PGD (Phosphogluconate Dehydrogenase (PGD))
    别名
    PGD (PGD 产品)
    别名
    6PGD ELISA Kit, 6-PGD ELISA Kit, 6-Pgd ELISA Kit, 6-pgd ELISA Kit, 6PGDH ELISA Kit, 6Pgd ELISA Kit, 6Pgdh ELISA Kit, 6pgdh ELISA Kit, A7 ELISA Kit, CG3724 ELISA Kit, Dmel\\CG3724 ELISA Kit, EG:87B1.4 ELISA Kit, N1 ELISA Kit, PGD ELISA Kit, Pdg ELISA Kit, l(1)2Dc ELISA Kit, l(1)2De ELISA Kit, l(1)A7 ELISA Kit, l(1)G0385 ELISA Kit, l(1)N1 ELISA Kit, l(1)N3[90] ELISA Kit, l(1)Pgd ELISA Kit, l(1)Pgd-A ELISA Kit, l35 ELISA Kit, pgd ELISA Kit, 0610042A05Rik ELISA Kit, AU019875 ELISA Kit, C78335 ELISA Kit, fi03d03 ELISA Kit, zgc:55585 ELISA Kit, wu:fi03d03 ELISA Kit, zgc:111848 ELISA Kit, 6-PGDH ELISA Kit, GND1b ELISA Kit, Pgd ELISA Kit, Cc2-27 ELISA Kit, phosphogluconate dehydrogenase ELISA Kit, Phosphogluconate dehydrogenase ELISA Kit, phosphogluconate dehydrogenase L homeolog ELISA Kit, 6-phosphogluconate dehydrogenase, decarboxylating ELISA Kit, 6-phosphogluconate dehydrogenase, decarboxylating-like ELISA Kit, PGD ELISA Kit, Pgd ELISA Kit, pgd.L ELISA Kit, pgd ELISA Kit, GND1a|GND1b ELISA Kit, Thal_1580 ELISA Kit, GY4MC1_3101 ELISA Kit, Odosp_2189 ELISA Kit, Marky_1024 ELISA Kit, gnd ELISA Kit, LOC101451876 ELISA Kit
    UniProt
    P52209
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