HAVCR1 ELISA 试剂盒
Quick Overview for HAVCR1 ELISA 试剂盒 (ABIN6720396)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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Supplier Product No.
- koa0529
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Supplier
- Rockland
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原理
- Mouse KIM1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse KIM1. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 2 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA Kit
HAVCR1 适用: 小鼠 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Tissue Homogenate, Urine
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Tissue Homogenate, Urine
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 猴 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 小鼠 Colorimetric Sandwich ELISA 0.078 ng/mL - 5 ng/mL Plasma, Serum, Urine
Hepatitis A Virus Cellular Receptor 1 (HAVCR1) ELISA KitHAVCR1 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate, Urine
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse KIM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, tissue homogenates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each mouse KIM1 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: Y22-T212
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
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别名
- KIM1
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背景
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Synonyms: HAVCR, HAVcr-1, Havcr1, HAVR1_HUMAN, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, KIM-1, T cell immunoglobin domain and mucin domain protein 1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell membrane protein 1, TIM, TIM-1, TIM1, TIMD-1, TIMD1
Background: KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al.(1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al.(2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al.(2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.
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基因ID
- 171283
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NCBI登录号
- NP_001160103
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UniProt
- Q3V033
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