ADA ELISA 试剂盒
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- 抗原 See all ADA ELISA试剂盒
- ADA (Adenosine Deaminase (ADA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Pleural Fluid, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: ada, ADA1, ADA 1, Adenosine aminohydrolase, Adenosine deaminase
Background: Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.
Gene Name: ADA
Production: Natural and recombinant human ADA. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: sf21, Immunogen sequence: A2-L363
- Top Product
- Discover our top product ADA ELISA Kit
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Adenosine Deaminase (ADA) ELISA Kit
ADA 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Adenosine Deaminase (ADA) ELISA KitADA 适用: 人 Colorimetric Sandwich ELISA 1.56 mIU/mL - 100 mIU/mL Serum, Tissue Homogenate, Urine
Adenosine Deaminase (ADA) ELISA KitADA 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Adenosine Deaminase (ADA) ELISA KitADA 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Adenosine Deaminase (ADA) ELISA KitADA 适用: 人 AA 2-363 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Pleural Fluid, Serum, Tissue Homogenate
Adenosine Deaminase (ADA) ELISA KitADA 适用: Pig Colorimetric Competition ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human ADA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, tissue homogenates or pleural fluid to each empty well. It is recommended that each human ADA standard solution and each sample be measured in duplicate.
ELISA Dilution: 15.6pg/mL-1000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all ADA ELISA试剂盒
- ADA (Adenosine Deaminase (ADA))
- 别名
- ADA (ADA 产品)
- 别名
- ADA-like ELISA Kit, xada ELISA Kit, ADA ELISA Kit, CG11994 ELISA Kit, Dmel\\CG11994 ELISA Kit, DrosADA ELISA Kit, dADA ELISA Kit, zgc:92028 ELISA Kit, adenosine deaminase ELISA Kit, adenosine deaminase S homeolog ELISA Kit, Adenosine deaminase ELISA Kit, ADA ELISA Kit, Ada ELISA Kit, ada.S ELISA Kit, ada ELISA Kit
- 基因ID
- 100
- NCBI登录号
- NP_000013
- UniProt
- P00813
- 途径
- Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process
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