MMP13 ELISA 试剂盒
Quick Overview for MMP13 ELISA 试剂盒 (ABIN6720388)
抗原
See all MMP13 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0275
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Supplier
- Rockland
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原理
- Human MMP13 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human total MMP13. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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SensoLyte® 520 MMP-13 Assay Kit
FRET Fluorometric
SensoLyte® Plus 520 MMP-13 Assay Kit Fluorimetric and Enhanced SelectivityFRET 适用: 人 Fluorometric
SensoLyte® 490 MMP-13 Assay KitFRET Fluorometric
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 兔 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 人 AA 20-471 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP13 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin) to each empty well. It is recommended that each human MMP13 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: L20-C471
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))
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别名
- MMP13
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背景
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Synonyms: CLG 3, CLG3, Collagenase 3, Collagenase3, MANDP1, Matrix metalloproteinase 13, Matrix metalloproteinase-13, MMP 13, MMP-13, Mmp13, MMP13_HUMAN, UMRCASE
Background: Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
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基因ID
- 4322
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NCBI登录号
- NP_002418
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UniProt
- P45452
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