MMP1 ELISA 试剂盒
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Quick Overview for MMP1 ELISA 试剂盒 (ABIN6720383)
抗原
See all MMP1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0266
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Supplier
- Rockland
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原理
- Human MMP-1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: F20-N469 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 20 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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SensoLyte® Plus 520 MMP-1 Assay Kit Fluorimetric and Enhanced Selectivity
FRET 适用: 人 Fluorometric
SensoLyte® 520 MMP-1 Assay KitFRET Fluorometric
SensoLyte® 490 MMP-1 Assay KitFRET Fluorometric
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 人 AA 20-469 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1) ELISA KitMMP1 适用: 犬 Colorimetric Sandwich ELISA 0.18 ng/mL - 12 ng/mL Plasma, Serum, Tissue Homogenate
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin) to each empty well. It is recommended that each human MMP-1 standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 6.5%, 7.6%, 5.8%
Inter-Assay Precision (CV%): 8.3%, 9.4%, 7.6% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
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别名
- MMP-1
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背景
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Background: Matrix metalloproteinase-1 (MMP-1) is also known as collagenase. Matrix metalloproteinases (MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1 (MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1 (interstitial collagenase), MMP9 (gelatinase B) and MMP12 (macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.
Gene Full Name: matrix metallopeptidase 1
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基因ID
- 4312
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NCBI登录号
- NP_001139410
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UniProt
- P03956
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