MMP8 ELISA 试剂盒
Quick Overview for MMP8 ELISA 试剂盒 (ABIN6720378)
抗原
See all MMP8 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0272
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Supplier
- Rockland
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原理
- Human MMP-8 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human total MMP-8. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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SensoLyte® 520 MMP-8 Assay Kit
FRET Fluorometric
SensoLyte® 490 MMP-8 Assay KitFRET Fluorometric
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 适用: 豚鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 适用: 豚鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) ELISA KitMMP8 适用: 大鼠 AA 21-466 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin) or saliva to each empty well. It is recommended that each human MMP-8 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: F21-G467
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
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别名
- MMP-8
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背景
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Synonyms: BB138268, CLG 1, CLG1, Collagenase 1, Collagenase 1 neutrophil, HNC, Matrix metallopeptidase 8, Matrix metalloproteinase 8 (neutrophil collagenase), Matrix metalloproteinase-8, MMP 8, MMP-8, Mmp8, MMP8_HUMAN, Neutrophil collagenase, PMNL CL, PMNL collagenase, PMNL-CL
Background: Matrix metalloproteinase 8(MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic cross reactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase(HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57 % identity with the deduced protein sequence for fibroblast collagenase with 72 % chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.
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基因ID
- 4317
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NCBI登录号
- NP_001291370
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UniProt
- P22894
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