ADAM12 ELISA 试剂盒
Quick Overview for ADAM12 ELISA 试剂盒 (ABIN6720375)
抗原
See all ADAM12 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0569
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Supplier
- Rockland
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原理
- Human ADAM12 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human ADAM12. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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ADAM Metallopeptidase Domain 12 (ADAM12) ELISA Kit
ADAM12 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase Domain 12 (ADAM12) ELISA KitADAM12 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
ADAM Metallopeptidase Domain 12 (ADAM12) ELISA KitADAM12 适用: 大鼠 AA 29-513 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
ADAM Metallopeptidase Domain 12 (ADAM12) ELISA KitADAM12 适用: 人 AA 29-513 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum, Urine
ADAM Metallopeptidase Domain 12 (ADAM12) ELISA KitADAM12 适用: 小鼠 AA 29-513 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human ADAM12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin) or urine to each empty well. We recommend that each human ADAM12 standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: CHO, Immunogen sequence: R29-S513
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))
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别名
- ADAM12
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背景
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Synonyms: ADA12_HUMAN, ADAM 12, ADAM metallopeptidase domain 12, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, MCMP, MCMPMltna, Meltrin-alpha, MLTN, MLTNA
Background: ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM(a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.
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基因ID
- 8038
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NCBI登录号
- NP_001275903
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UniProt
- O43184
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途径
- EGFR Signaling Pathway
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