CD163 ELISA 试剂盒
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北京 101111
Quick Overview for CD163 ELISA 试剂盒 (ABIN6720374)
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See all CD163 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.78 ng/mL
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Supplier Product No.
- koa0658
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Supplier
- Rockland
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原理
- Mouse CD163 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: V39-T1045 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 20 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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CD163 (CD163) ELISA Kit
CD163 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
CD163 (CD163) ELISA KitCD163 适用: Pig Colorimetric Sandwich ELISA 93.75 ng/mL - 6000 ng/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
CD163 (CD163) ELISA KitCD163 适用: 人 AA 41-1045 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma (heparin), Serum
CD163 (CD163) ELISA KitCD163 适用: 小鼠 AA 39-1045 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma (heparin), Serum
CD163 (CD163) ELISA KitCD163 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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说明
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Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse CD163 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin) to each empty well. We recommend that each mouse CD163 standard solution and each sample is measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 7.8%, 4.3%, 7.1%
Inter-Assay Precision (CV%): 8.5%, 4.7%, 7.9% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CD163
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别名
- CD163
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背景
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Background: CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.
Gene Full Name: CD163 antigen
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基因ID
- 93671
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NCBI登录号
- NP_444324
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UniProt
- Q2VLH6
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