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CYR61 ELISA 试剂盒

This 人 CYR61 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 CYR61 in Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum 和 Urine.
Rockland
产品编号 ABIN6720366
Supplier Product No.: koa0700
发货至: 中国

Quick Overview for CYR61 ELISA 试剂盒 (ABIN6720366)

抗原

See all CYR61 ELISA试剂盒
CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

适用

  • 6
  • 3
  • 1
  • 1

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
  • 最低检测浓度

    31.2 pg/mL

    Supplier Product No.

    koa0700

    Supplier

    Rockland

    原理

    Human CYR61 - CCN1 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human CYR61. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CYR61 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine or human milk to each empty well. It is recommended that each human CYR61 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: CHO, Immunogen sequence: A22-D381

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all CYR61 ELISA试剂盒

    CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

    别名

    CYR61/CCN1

    背景

    Synonyms: 3CH61, CCN 1, CCN family member 1, CCN1, CCN1, CYR 61, Cyr61, CYR61 protein, CYR61 protein precursor, CYR61 protein precursor, CYR61_HUMAN, Cysteine rich angiogenic inducer 61, Cysteine rich heparin binding protein 61, Cysteine-rich angiogenic inducer 61, GIG 1, GIG1, GIG1 protein, IBP-10, IGF-binding protein 10, Igfbp 10, IGFBP-10, IGFBP10, Insulin like growth factor binding protein 10, Insulin-like growth factor-binding protein 10, Protein CYR61, Protein GIG1

    Background: Cysteine-rich angiogenic inducer 61(CYR61) or CCN family member 1(CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.

    基因ID

    3491

    NCBI登录号

    NP_001545

    UniProt

    O00622

    途径

    Positive Regulation of Endopeptidase Activity, Growth Factor Binding
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