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IL-5 ELISA 试剂盒

This 人 IL-5 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 IL-5.
Rockland
产品编号 ABIN6720357
Supplier Product No.: koa0223
发货至: 中国

Quick Overview for IL-5 ELISA 试剂盒 (ABIN6720357)

抗原

See all IL-5 (IL5) ELISA试剂盒
IL-5 (IL5) (Interleukin 5 (IL5))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

7.8 pg/mL - 500 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
  • 最低检测浓度

    7.8 pg/mL

    Supplier Product No.

    koa0223

    Supplier

    Rockland

    原理

    Human IL-5 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human IL-5. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 2 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human IL-5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human IL-5 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: SF21, Immunogen sequence: I20-S134

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all IL-5 (IL5) ELISA试剂盒

    IL-5 (IL5) (Interleukin 5 (IL5))

    别名

    IL-5

    背景

    Synonyms: B cell differentiation factor I, B-cell differentiation factor I, Colony stimulating factor eosinophil, EDF, Eosinophil differentiation factor, IL 5, IL-5, IL5, IL5_HUMAN, Interleukin 5, Interleukin 5 (colony stimulating factor eosinophil), Interleukin-5 Interleukin5, T cell replacing factor, T-cell replacing factor, TRF

    Background: Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70 % amino acid sequence homology.

    基因ID

    3567

    NCBI登录号

    NP_000870

    UniProt

    P05113

    途径

    JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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