IL18BP ELISA 试剂盒
Quick Overview for IL18BP ELISA 试剂盒 (ABIN6720356)
抗原
See all IL18BP ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 93.7 pg/mL
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Supplier Product No.
- koa0758
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Supplier
- Rockland
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原理
- Human IL-18BP Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human IL-18BP. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Interleukin 18 Binding Protein (IL18BP) ELISA Kit
IL18BP 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 大鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 人 AA 31-194 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 人 Colorimetric Sandwich ELISA 94-6000 pg/mL Cell Lysate, Plasma, Serum
Interleukin 18 Binding Protein (IL18BP) ELISA KitIL18BP 适用: 人 Colorimetric Sandwich ELISA 20-18000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/m human IL-18BP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human IL-18BP standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: T31-G194
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IL18BP (Interleukin 18 Binding Protein (IL18BP))
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别名
- IL-18BP
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背景
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Synonyms: I18BP_HUMAN, IL-18BP, IL18BP, Interleukin-18-binding protein, Tadekinig-alfa
Background: Interleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease.
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基因ID
- 10068
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NCBI登录号
- NP_001034748
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UniProt
- O95998
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