APOA1 ELISA 试剂盒
Quick Overview for APOA1 ELISA 试剂盒 (ABIN6720352)
抗原
See all APOA1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.12 ng/mL
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Supplier Product No.
- koa0879
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Supplier
- Rockland
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原理
- Human APOA1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural human APOA1. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 50 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Apolipoprotein A-I (APOA1) ELISA Kit
APOA1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Bronchoalveolar Lavage, Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 人 Colorimetric Sandwich ELISA 15.6 ng/mL - 500 ng/mL Plasma, Serum, Urine
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 兔 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: Pig Colorimetric Sandwich ELISA 1.563 μg/mL - 90 μg/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein A-I (APOA1) ELISA KitAPOA1 适用: 犬 Colorimetric Competition ELISA 28.12 ng/mL - 1800 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL human APOA1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human APOA1 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: D25-Q267
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- APOA1 (Apolipoprotein A-I (APOA1))
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别名
- APOA1
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背景
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Synonyms: Apo AI, Apo-AI, ApoA I, ApoA-I, APOA1, Apolipoprotein A I, Apolipoprotein A-I, Apolipoprotein A1, Apolipoprotein AI, ApolipoproteinAI, Brp14
Background: Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene. This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterol acyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein.
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基因ID
- 335
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NCBI登录号
- NP_000030
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UniProt
- P02647
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途径
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
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