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ACVA ELISA 试剂盒

This 小鼠 ACVA ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 ACVA.
Rockland
产品编号 ABIN6720345
Supplier Product No.: koa0142
发货至: 中国

Quick Overview for ACVA ELISA 试剂盒 (ABIN6720345)

抗原

See all ACVA ELISA试剂盒
ACVA (Activin A (ACVA))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
  • 最低检测浓度

    15.6 pg/mL

    Supplier Product No.

    koa0142

    Supplier

    Rockland

    原理

    Mouse Activin A Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse Activin A. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse Activin A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. It is recommended that each mouse Activin A standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: CHO, Immunogen sequence: G311-S426

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all ACVA ELISA试剂盒

    ACVA (Activin A (ACVA))

    别名

    Activin A

    背景

    Synonyms: Activin beta A chain, erythroid differentiation factor, erythroid differentiation protein, follicle stimulating hormone releasing protein, FSH releasing

    Background: Activin A is a homodimer of 14 kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.

    基因ID

    16323

    NCBI登录号

    NP_032406

    UniProt

    Q04998

    途径

    Hormone Transport, Peptide Hormone Metabolism
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