MIA ELISA 试剂盒
Quick Overview for MIA ELISA 试剂盒 (ABIN6720344)
抗原
See all MIA ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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Supplier Product No.
- koa0576
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Supplier
- Rockland
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原理
- Human MIA Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human MIA. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Melanoma Inhibitory Activity (MIA) ELISA Kit
MIA 适用: 小鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Melanoma Inhibitory Activity (MIA) ELISA KitMIA 适用: 人 AA 25-131 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MIA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. It is recommended that each human MIA standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: G25-131Q
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MIA (Melanoma Inhibitory Activity (MIA))
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别名
- MIA
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背景
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Synonyms: CD RAP, melanoma inhibitory activity, Melanoma inhibitory activity protein, Melanoma-derived growth regulatory protein, Mia, MIA_HUMAN
Background: Melanoma Inhibiting Activity(MIA), also known as cartilage-derived retinoic acid-sensitive protein(CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain(1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .
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基因ID
- 8190
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NCBI登录号
- NP_001189482
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UniProt
- Q16674
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