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IGFBPI ELISA 试剂盒

This 人 IGFBPI ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 IGFBPI in Cell Culture Supernatant, Plasma (EDTA - heparin), Serum 和 Urine.
Rockland
产品编号 ABIN6720316
Supplier Product No.: koa0199
发货至: 中国

Quick Overview for IGFBPI ELISA 试剂盒 (ABIN6720316)

抗原

See all IGFBPI ELISA试剂盒
IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
  • 最低检测浓度

    31.2 pg/mL

    Supplier Product No.

    koa0199

    Supplier

    Rockland

    原理

    Human IGFBP-1 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human IGFBP-1. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 1 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IGFBP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. It is recommended that each human IGFBP-1 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: A26-N259

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all IGFBPI ELISA试剂盒

    IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

    别名

    IGFBP-1

    背景

    Synonyms: AFBP, hIGFBP-1, IBP1, IGF-binding protein 1, IGF-BP25, Insulin-like growth factor-binding protein 1, Placental protein 12, PP12

    Background: IGFBP1 is also known as amniotic fluid binding protein(AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein(IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25KDa.

    基因ID

    3484

    NCBI登录号

    NP_000587

    UniProt

    P08833

    途径

    Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding
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