Relaxin 1 ELISA 试剂盒
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Quick Overview for Relaxin 1 ELISA 试剂盒 (ABIN6720306)
抗原
See all Relaxin 1 (RLN1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 78 pg/mL
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Supplier Product No.
- koa0722
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Supplier
- Rockland
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原理
- Human Relaxin 1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: R162-Cys185 (A) & K26-S53 (B) -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Relaxin 1 (RLN1) ELISA Kit
RLN1 适用: 兔 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 犬 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 大鼠 Colorimetric Sandwich ELISA 2.4 pg/mL - 150 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 小鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 大鼠 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 人 Colorimetric Competition ELISA 3.2 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: Cow Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
Relaxin 1 (RLN1) ELISA KitRLN1 适用: Pig Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Relaxin 1 (RLN1) ELISA KitRLN1 适用: 兔 Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Plasma, Serum
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human Relaxin 1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each human Relaxin 1 standard solution and each sample is measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 7.6%, 4.6%, 4.2%
Inter-Assay Precision (CV%): 7.9%, 5.4%, 4.6% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Relaxin 1 (RLN1)
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别名
- Relaxin 1
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背景
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Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to human chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.
Gene Full Name: relaxin 1
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基因ID
- 6013
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NCBI登录号
- NP_008842
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UniProt
- P04808
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