REG4 ELISA 试剂盒
Quick Overview for REG4 ELISA 试剂盒 (ABIN6720301)
抗原
See all REG4 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0765
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Supplier
- Rockland
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原理
- Human REG-4 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human REG-4. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Regenerating Islet-Derived Family, Member 4 (REG4) ELISA Kit
REG4 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Regenerating Islet-Derived Family, Member 4 (REG4) ELISA KitREG4 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Regenerating Islet-Derived Family, Member 4 (REG4) ELISA KitREG4 适用: 小鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Regenerating Islet-Derived Family, Member 4 (REG4) ELISA KitREG4 适用: 人 AA 23-158 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human REG-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human REG-4 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: D23-P158
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))
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别名
- REG-4
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背景
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Synonyms: Gastrointestinal secretory protein, GISP, REG IV, REG like protein, REG-4, REG-like protein, Reg4, REG4_HUMAN, Regenerating gene type IV, Regenerating islet derived family member 4, Regenerating islet derived protein 4 precursor, Regenerating islet-derived protein 4, Regenerating islet-derived protein IV, REGIV, RELP
Background: Regenerating islet-derived protein 4 is a protein that in humans is encoded by the REG4 gene. It is mapped to 1p12. REG-4 gene contains 6 exons and that the exon structure is preserved among members of the REG gene family. This gene is able to maintain carbohydrate recognition activity in an acidic environment. REG-4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis, and most of the colorectal tumors overexpressing REG-4 are mucinous tumors or neuroendocrine tumors.
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基因ID
- 83998
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NCBI登录号
- NP_001152824
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UniProt
- Q9BYZ8
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