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ANGPTL4 ELISA 试剂盒

This 人 ANGPTL4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 ANGPTL4.
Rockland
产品编号 ABIN6720260
Supplier Product No.: koa0590
发货至: 中国

Quick Overview for ANGPTL4 ELISA 试剂盒 (ABIN6720260)

抗原

See all ANGPTL4 ELISA试剂盒
ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

625 pg/mL - 40000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    625 pg/mL

    Supplier Product No.

    koa0590

    Supplier

    Rockland

    原理

    Human ANGPTL4 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human ANGPTL4. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 20 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40000pg/mL, 20000pg/mL, 10000pg/mL, 5000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human ANGPTL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ANGPTL4 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: L165-S406

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all ANGPTL4 ELISA试剂盒

    ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))

    别名

    ANGPTL4

    背景

    Synonyms: 425O18 1, Angiopoietin like 4, Angiopoietin related protein 4, ANGPT L2 , ANGPT L4, ANGPTL2, ANGPTL4 , Fasting induced adipose factor , FIAF, Fibrinogen/angiopoietin related protein, Hepatic fibrinogen/angiopoietin related protein , HFARP, Major histocompatibility complex region NG27, NL2, Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein , PGAR, pp1158 , PPARG angiopoietin related protein , PSEC0166 , Secreted protein Bk89, Weakly similar to angiopoietin 1

    Background: Angiopoietin-related protein 4(Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

    基因ID

    51129

    NCBI登录号

    NP_001034756

    UniProt

    Q9BY76

    途径

    Regulation of Lipid Metabolism by PPARalpha
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